Difference between revisions of "Part:BBa K743004:Design"

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===Design Notes===
 
===Design Notes===
  
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Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from Ecor1)
+
Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from EcoR1)
 
No mutation was done so that complete homology with Synechocystis chromosome is achieved
 
No mutation was done so that complete homology with Synechocystis chromosome is achieved
  
 
===Source===
 
===Source===
−
 
−
BBa_B0015 terminator sequence was obtained from the registry and ligated with Synechocystis genomic region sll0337 by Gibson assembly
 
  
 
===References===
 
===References===

Latest revision as of 00:32, 23 September 2012

psb1C3_IntK plasmid with RFP under psaAB promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2752
    Illegal XhoI site found at 3249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2752
    Illegal AgeI site found at 1270
    Illegal AgeI site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from EcoR1) No mutation was done so that complete homology with Synechocystis chromosome is achieved

Source

References