Difference between revisions of "Part:BBa K823040"
 
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<partinfo>BBa_K823040 short</partinfo>
 
<partinfo>BBa_K823040 short</partinfo>
  
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This part reverses the positive Input of the pBad Promoter induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.
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[[Image:Inverter_pellets.jpg|600px]]
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This part reverses the positive Input of the P<sub>''BAD''</sub> Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the ''lacZα'' Reporter, by the translational inhibitory action of the small RNA RyhB to uof<sub>CGU</sub> translationally fused to lacZα.
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[[Image:Inverter_graph.png|300px]][[Image:Inverter Construct.png|300px]]
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This Image depicts the β-Galactosidase assay done with this part. It converts the positive Input of the P<sub>''BAD''</sub> promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).
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[[File:LMU Inverter graph|600px]]>
 
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 13:41, 26 September 2012

Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha

Inverter pellets.jpg

This part reverses the positive Input of the PBAD Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZα Reporter, by the translational inhibitory action of the small RNA RyhB to uofCGU translationally fused to lacZα.

Inverter graph.pngInverter Construct.png

This Image depicts the β-Galactosidase assay done with this part. It converts the positive Input of the PBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]