Difference between revisions of "Part:BBa K812130"
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<partinfo>BBa_K812130 short</partinfo> | <partinfo>BBa_K812130 short</partinfo> | ||
− | Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems | + | <html> |
+ | </br> | ||
+ | <b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b> | ||
+ | </br></br> | ||
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+ | Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and the J04450 is replaced by the mCitrine <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>, it is ready for immediate use in Xenopus. | ||
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+ | </br>This part was characterized and the result is below. | ||
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+ | Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="500px" /><br/> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 16:56, 30 September 2012
Citrine reporter with a Kozak sequence for expression in Xenopus
*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812030. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***
Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is BBa_K812000 and the J04450 is replaced by the mCitrine BBa_K812030, it is ready for immediate use in Xenopus.
This part was characterized and the result is below.
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
Sequence and Features