Difference between revisions of "Part:BBa K812133"

 
(rollbackEdits.php mass rollback)
 
(7 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K812133 short</partinfo>
+
<partinfo>BBa_K812031 short</partinfo>
 +
 
 +
<html>
 +
</br>
 +
<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
 +
</br></br>
 +
 
 +
</br>This part was  created with the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>  and J04450 was replaced by sfGFP (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>). This plasmid express sfGFP into Xenopus: <br/>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/>
 +
<p>The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into <i>Xenopus</i> it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.<br/>
  
−
Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts. Ready for immediate use in Xenopus.
+
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 10: Line 19:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K812133 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K812031 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K812133 parameters</partinfo>
+
<partinfo>BBa_K812031 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 16:18, 10 May 2013

sfGFP with kozak sequence for eukaryotic expression


*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***


This part was created with the plasmid backbone BBa_K812000 and J04450 was replaced by sfGFP (BBa_K812031). This plasmid express sfGFP into Xenopus:
perdu

The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into Xenopus it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19