Difference between revisions of "Part:BBa K863121:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein. | |
+ | |||
+ | Primers that were used to make this BioBrick: | ||
+ | {| | ||
+ | |GFP_Frei||TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT | ||
+ | |- | ||
+ | |GFP_His6_compl||CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG | ||
+ | |} | ||
===Source=== | ===Source=== |
Latest revision as of 22:13, 26 September 2012
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 742 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653
Design Notes
This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein.
Primers that were used to make this BioBrick:
GFP_Frei | TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT |
GFP_His6_compl | CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG |