Difference between revisions of "Part:BBa K863121:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K863121 short</partinfo>
 
<partinfo>BBa_K863121 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Added Freiburg-restriction-sites for in frame assembly.
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This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein.
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+
  
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Primers that were used to make this BioBrick:
 +
{|
 +
|GFP_Frei||TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT
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|-
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|GFP_His6_compl||CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG
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|}
  
 
===Source===
 
===Source===

Latest revision as of 22:13, 26 September 2012

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


Design Notes

This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein.

Primers that were used to make this BioBrick:

GFP_Frei TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT
GFP_His6_compl CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG

Source

BBa_I13522

References