Difference between revisions of "Part:BBa K818300"

Latest revision as of 11:53, 25 September 2012

Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

alsT is a promoter in B. subtilis repressed by TnrA. TnrA, in turn, is only active when glutamine synthetase (GS) is actively converting NH4 to glutamine. This in turn is regulated by cell growth, not the amount of NH4/NH3+ present. However, in one article it was observed that GS is also active under conditions of nitrogen limitation. If the medium contained the precise amount of glutamine necessary to support the initial growth phase, then the glutamine would be depleted at the sensing phase. GS would activate to try and create more glutamine. GS would only deactivate when the amount of extracellular NH4/NH3+ reached a level to remove the lack of nitrogen as factor limiting further growth.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 465
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 465
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 465
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 465
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 465
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K818300 short</partinfo>
-Promoter alsT is a promoter that is repressed by tnrA in the presence of low ammonium by B. subtilis. tnrA is a promoter that responds to ammonium availability in the environment. At low availbility tnrA is activated thus repressing alsT. At high availbility of ammonium (produced by rotten meat), tnrA will be deactivated and alsT will be active.
+alsT is a promoter in B. subtilis repressed by TnrA. TnrA, in turn, is only active when glutamine synthetase (GS) is actively converting NH4 to glutamine. This in turn is regulated by cell growth, not the amount of NH4/NH3+ present. However, in one article it was observed that GS is also active under conditions of nitrogen limitation. If the medium contained the precise amount of glutamine necessary to support the initial growth phase, then the glutamine would be depleted at the sensing phase. GS would activate to try and create more glutamine. GS would only deactivate when the amount of extracellular NH4/NH3+ reached a level to remove the lack of nitrogen as factor limiting further growth.
 <!-- Add more about the biology of this part here