Difference between revisions of "Part:BBa K769015"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K769015 short</partinfo> | <partinfo>BBa_K769015 short</partinfo> | ||
− | Promoter(high)-RBS-lux CDBFEG-DT | + | Promoter(high)-RBS-lux CDBFEG-DT (lux operon from <i>Photobacterium phosphoreum</I> NCMB844) |
− | + | <BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png | |
+ | <BR> | ||
+ | <BR> | ||
+ | <BR> This part is constructed from our cloned lux operon, BBa_K769010. | ||
+ | <BR> The <i>luxCDBEFG</i> is missing a <i>luxA</i> gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 07:06, 4 October 2012
Promoter(high)-RBS-lux CDBFEG-DT
Promoter(high)-RBS-lux CDBFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)
This part is constructed from our cloned lux operon, BBa_K769010.
The luxCDBEFG is missing a luxA gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 505
Illegal NheI site found at 3669 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4131
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 790
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 684
Illegal BsaI.rc site found at 1366