Difference between revisions of "Part:BBa K812012:Experience"
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===Applications of BBa_K812012=== | ===Applications of BBa_K812012=== | ||
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| + | The Nevada 2014 iGEM team used TIR1 in their yeast protein degradation project. We cloned TIR1 behind a constitutive yeast promoter (pADH1). We added a 9xMyc tag to help verify expression. After integrating our construct into W303 yeast cells, we were able to verify expression via western blot. | ||
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| + | The results can be seen on our team page: [http://2014.igem.org/Template:Nevada/TheAuxinSystem TIR1 Expression by Western Blot] | ||
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| + | <html> | ||
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| + | <img src="http://www.matthewbrianhawn.com/jpg/western_blot_tir1"> | ||
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| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:05, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K812012
The Nevada 2014 iGEM team used TIR1 in their yeast protein degradation project. We cloned TIR1 behind a constitutive yeast promoter (pADH1). We added a 9xMyc tag to help verify expression. After integrating our construct into W303 yeast cells, we were able to verify expression via western blot.
The results can be seen on our team page: [http://2014.igem.org/Template:Nevada/TheAuxinSystem TIR1 Expression by Western Blot]
User Reviews
UNIQded9bf59965ba8aa-partinfo-00000001-QINU UNIQded9bf59965ba8aa-partinfo-00000002-QINU