Difference between revisions of "Part:BBa K812012"

 
 
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<partinfo>BBa_K812012 short</partinfo>
 
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OsTiRI is an ubuquitinase E3 that recognise and trigger the ubiquitination of every AID tagged protein. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
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OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
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This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.  
  
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This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.  
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This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.
  
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This part contains 3 myc domain for protein staining using antiMyc antibodies
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This part can be micro-injected using pSC2+ ( <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>) or related plasmid as a backbone.
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<img src="https://static.igem.org/mediawiki/parts/5/5a/Degron1.jpg" alt="perdu" width="1000px" /> <br/><br/>
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:10, 27 October 2012

OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.

This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.

This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.

This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone. perdu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
    Illegal NotI site found at 287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1648
    Illegal XhoI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79