Difference between revisions of "Part:BBa K777113:Design"
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===Design Notes===
 
===Design Notes===
 
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
 
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
−
**Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG
+
**Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3'
−
**Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
+
**Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 08:44, 19 September 2012

motA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 772


Design Notes

  • motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3'
    • Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References