Difference between revisions of "Part:BBa K731500:Design"

(→‎Design Notes)
(→‎Source)
 
(6 intermediate revisions by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
This part is composed of K731300 + a ''tac'' promoter followed by a
+
This part is composed of [https://parts.igem.org/Part:BBa_K731300 K731300] + a ''tac'' promoter followed by a
 
''lac'' operator. ''lac'' operator sequence corresponds to plasmid pET21b one.
 
''lac'' operator. ''lac'' operator sequence corresponds to plasmid pET21b one.
 +
 
''tac'' promoter and ''lac'' operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
 
''tac'' promoter and ''lac'' operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
  
 
===Source===
 
===Source===
  
−
K731300 part + Ptac & lacO from pET21b
+
[https://parts.igem.org/Part:BBa_K731300 BBa_K731300] part + P''tac'' & lacO from pET21b
  
 
===References===
 
===References===

Latest revision as of 10:30, 17 September 2012

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of K731300 + a tac promoter followed by a lac operator. lac operator sequence corresponds to plasmid pET21b one.

tac promoter and lac operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.

Source

BBa_K731300 part + Ptac & lacO from pET21b

References