Difference between revisions of "Part:BBa K784015:Design"

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===Design Notes===
 
===Design Notes===
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Hila, update this.
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
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The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other. <br>
  
  

Latest revision as of 15:59, 21 September 2012

Phage Lambda fragment 1 - head proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
    Illegal BamHI site found at 5526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
    Illegal AgeI site found at 4751
    Illegal AgeI site found at 4900
    Illegal AgeI site found at 6582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2418
    Illegal SapI.rc site found at 6510


Design Notes

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1
The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp

References