Difference between revisions of "Part:BBa K733010:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K733010 short</partinfo> | <partinfo>BBa_K733010 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | None | |
+ | ===Source=== | ||
+ | We digest ''Ptms'' and RBS+''ydcD'' and ligate them. | ||
− | + | Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF. | |
− | + | Insert: RBS+''ydcD''. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | |
===References=== | ===References=== |
Latest revision as of 16:34, 26 September 2012
Ptms+RBS+ydcD.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
We digest Ptms and RBS+ydcD and ligate them.
Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification.