Difference between revisions of "Part:BBa K733010:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K733010 short</partinfo>
 
<partinfo>BBa_K733010 short</partinfo>
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===Design Notes===
 
===Design Notes===
Not available at this moment.
+
None
  
 +
===Source===
  
 +
We digest ''Ptms'' and RBS+''ydcD'' and ligate them.
  
===Source===
+
Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF.
  
Not available at this moment.
+
Insert: RBS+''ydcD''. Enzyme used: XbaI and PstI-HF. Purified by gel purification.
  
 
===References===
 
===References===

Latest revision as of 16:34, 26 September 2012

Ptms+RBS+ydcD.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

We digest Ptms and RBS+ydcD and ligate them.

Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.

Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification.

References