Difference between revisions of "Part:BBa K733003:Design"

 
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<partinfo>BBa_K733003 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Not available at this moment.
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To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in ''B. subtilis'' is designed, aiming at adding RBS directly to the upstream of ''ydcD'' gene through PCR.
  
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===Source===
  
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We obtain this part from the genomic DNA of ''Bacillus subtilis 168'' via PCR amplification.
  
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===Source===
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Forward primer: 5’-AAAGGAGGTGTATGTTTTTTGTCTG-3’ (Prefix not shown)
  
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Not available at this moment.
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Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)
  
 
===References===
 
===References===

Latest revision as of 19:47, 26 September 2012

RBS+ydcD: antitoxin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in B. subtilis is designed, aiming at adding RBS directly to the upstream of ydcD gene through PCR.

Source

We obtain this part from the genomic DNA of Bacillus subtilis 168 via PCR amplification.

Forward primer: 5’-AAAGGAGGTGTATGTTTTTTGTCTG-3’ (Prefix not shown)

Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)

References