Difference between revisions of "Part:BBa K929001:Design"

 
(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929001 short</partinfo>
 
<partinfo>BBa_K929001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS:  
+
Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS: <br>
−
ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG  
+
ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG <br>
−
 
+
−
Primer (reverse, complement)with AgeI, SpeI and PstI recognition site:
+
−
GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC
+
−
 
+
−
[[Image: UP12_biobrick1.jpg]]
+
−
 
+
  
 +
Primer (reverse, complement)with AgeI, SpeI and PstI recognition site: <br>
 +
GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC <br>
  
 +
[[Image: UP12_biobrick1.jpg|800px]]
  
 
===Source===
 
===Source===

Latest revision as of 06:22, 17 September 2012

AID without NES, with NLS and Kozak sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal SapI site found at 209


Design Notes

Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS:
ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG

Primer (reverse, complement)with AgeI, SpeI and PstI recognition site:
GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC

UP12 biobrick1.jpg

Source

AID BioBrick (BBa_K103001) and self-designed primers

References