Difference between revisions of "Part:BBa K861162"
(3 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
<partinfo>BBa_K861162 short</partinfo> | <partinfo>BBa_K861162 short</partinfo> | ||
− | + | This is the coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcription dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of the binding site in the CRP regulated promoter. This part is used to prevent the leaky expression of the promoter we designed. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 15:25, 22 September 2012
Constitutively expresses Crp
This is the coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcription dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of the binding site in the CRP regulated promoter. This part is used to prevent the leaky expression of the promoter we designed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]