Difference between revisions of "Part:BBa K861013"
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<partinfo>BBa_K861013 short</partinfo> | <partinfo>BBa_K861013 short</partinfo> | ||
− | To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins | + | |
+ | To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadD. | ||
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Latest revision as of 13:41, 22 September 2012
IPTG induced FadD
To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]