Difference between revisions of "Part:BBa K784000"
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<partinfo>BBa_K784000 short</partinfo> | <partinfo>BBa_K784000 short</partinfo> | ||
| − | This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎ | + | This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎<br> |
| − | The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the | + | The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the [https://parts.igem.org/Arkin_JCA_PNPAssay PNP (para-nitrophenol phosphate) hydrolysis assay] that has been ‎made by Arkin Lab group in 2006.‎<br> |
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes. ‎ | The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes. ‎ | ||
Latest revision as of 15:21, 13 September 2012
pT7+RBS+Alkaline Phosphatase (pHO)+Terminator
This part consists of T7 RNA polymerase induced promoter followed by an RBS and the Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.
The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been made by Arkin Lab group in 2006.
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and therefore can be excised out by these enzymes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1512
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1512
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1512
Illegal XhoI site found at 24 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1512
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1512
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 798 - 1000COMPATIBLE WITH RFC[1000]