Difference between revisions of "Part:BBa K823024"
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<partinfo>BBa_K823024 short</partinfo> | <partinfo>BBa_K823024 short</partinfo> | ||
− | This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pXT. | + | |
+ | This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. | ||
+ | This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pXT. | ||
+ | |||
+ | [[Image:LMU-Munich-PSBBs4S-Pxyl.png|600px]] | ||
+ | |||
+ | ==CAUTION: The submitted BioBrick can somehow not be transformed into ''Bacillus subtilis''. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!== | ||
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] | This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] |
Latest revision as of 17:49, 23 September 2012
pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis
This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose.
This backbone is a BioBricked version of the B. subtilis vector pXT.
CAUTION: The submitted BioBrick can somehow not be transformed into Bacillus subtilis. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]
Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré et al.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4779 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal BamHI site found at 4757
Illegal XhoI site found at 1011
Illegal XhoI site found at 1929 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4773
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4773
Plasmid lacks a suffix.
Illegal XbaI site found at 4788
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1893
Illegal NgoMIV site found at 3032 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2856
Illegal SapI.rc site found at 605