Difference between revisions of "Part:BBa K861023"
 
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<partinfo>BBa_K861023 short</partinfo>
 
<partinfo>BBa_K861023 short</partinfo>
  
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FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE have far better efficiency compared to FadE in E.coli. The original S-FadE gene contains PstI. This parts has already been point mutated (The G at 1983bp of FadE was mutated to A).
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FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE has far better efficiency compared to FadE in E.coli. The original S-FadE gene contains a PstI restriction site. This part has already been point mutated (The G at 1983bp of FadE was mutated to A).
  
 
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Latest revision as of 13:49, 22 September 2012

S-FadE with a RBS and a terminator

FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE has far better efficiency compared to FadE in E.coli. The original S-FadE gene contains a PstI restriction site. This part has already been point mutated (The G at 1983bp of FadE was mutated to A).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 378
    Illegal AgeI site found at 2568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535
    Illegal SapI.rc site found at 823
    Illegal SapI.rc site found at 1084