Difference between revisions of "Part:BBa K861060"

Latest revision as of 14:18, 22 September 2012

PfadR, synthetic promoter with tandem FadR binding site

At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is placed downstream of promoter BBa_J23110 with a 3bp-overlap. We tested its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K861060 short</partinfo>
-At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap.
+At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is placed downstream of promoter BBa_J23110 with a 3bp-overlap.
-We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.
+We tested its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.