Difference between revisions of "Part:BBa K823030:Experience"
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===Applications of BBa_K823030=== | ===Applications of BBa_K823030=== | ||
| − | We used the promtoer P''cotYZ'' for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025 BBa_K823025]]). | + | We used the promtoer P''cotYZ'' for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence. |
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| + | This part was used to display fusion proteins on ''B. subtilis'' spores. | ||
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| + | These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] | ||
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| + | [[Image:Activity PcotYZ PcotV.png|thumb|center|400px]] | ||
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 14:15, 7 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K823030
We used the promtoer PcotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.
This part was used to display fusion proteins on B. subtilis spores.
These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033
User Reviews
UNIQ03f36155ae4069b2-partinfo-00000000-QINU UNIQ03f36155ae4069b2-partinfo-00000001-QINU