Difference between revisions of "Part:BBa K268000:Experience"

Latest revision as of 17:09, 24 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K268000

User Reviews

Earthnut

WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.

An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.4 kbp and one at ~1.4 kbp (3.4 kbp + 1.4 kbp = 4.8 kbp).

TU_MUNICH 2012

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UNIQb67f96176fa67cfa-partinfo-00000001-QINU UNIQb67f96176fa67cfa-partinfo-00000002-QINU

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 |-
 |width='10%'|
-<partinfo>BBa_K268000 AddReview 1</partinfo>
+<partinfo>BBa_K268000 AddReview 0</partinfo>
 <I>Earthnut</I>
 |width='60%' valign='top'|
 <span style="color: red"> WARNING: </span>THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.
-An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.3&nbbsp;kbp and one at 1.4&nbsp;kbp.
+An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.4&nbsp;kbp and one at ~1.4&nbsp;kbp (3.4&nbsp;kbp&nbsp;+&nbsp;1.4&nbsp;kbp&nbsp;=&nbsp;4.8&nbsp;kbp).
+[[Image:TUM12 20120906 anal gel P674-677x.jpg|300px]]
 '''TU_MUNICH 2012'''