Difference between revisions of "Part:BBa K861030"
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<partinfo>BBa_K861030 short</partinfo> | <partinfo>BBa_K861030 short</partinfo> | ||
| − | For | + | For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and a transporter—FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 14:04, 22 September 2012
FadB, gene for fatty acid degradation from E.coli K12
For E.coli to degrade fatty acid, at least four enzymes – FadA, FadB, FadD, FadE and a transporter—FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The following cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 150
Illegal BglII site found at 297 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]