Difference between revisions of "Part:BBa K741019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | If a lambda phage infects a bacteria, it will express protein CI and Cro. The CI will activate the promoter pRM and the anticro and the lysis(for E7 colicin) will be produced. The anticro is the antisense RNA of cro and can repress the expression of cro. The lysis can destroy the inner membrane and activate the OMPLA(Outer Membrane Phospholipase, an enzyme in many Gram-negative bacteria) to destroy the outer membrane. Then the host infected and the lambda phage will die. | |
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===Source=== | ===Source=== | ||
Latest revision as of 18:48, 25 September 2012
pRM-anticro-T-pRM-RBS-lysis-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
If a lambda phage infects a bacteria, it will express protein CI and Cro. The CI will activate the promoter pRM and the anticro and the lysis(for E7 colicin) will be produced. The anticro is the antisense RNA of cro and can repress the expression of cro. The lysis can destroy the inner membrane and activate the OMPLA(Outer Membrane Phospholipase, an enzyme in many Gram-negative bacteria) to destroy the outer membrane. Then the host infected and the lambda phage will die.
Source
BBa_K741016 BBa_K741014