Difference between revisions of "Part:BBa K731480:Design"

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===Design Notes===
 
===Design Notes===
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This part was created by Gibson assembly starting from K731400. sfGFP was amplified by PCR from psB1A2. CysE and sfGFP are separated by a short nucleotide linker that encodes for the amino acidic sequence GSGSGS.
+
This part was created by Gibson assembly starting from K731400. sfGFP was amplified by PCR from psB1A2. CysDes and sfGFP are separated by a short nucleotide linker that encodes for the amino acidic sequence GSGSGS.
  
 
===Source===
 
===Source===
  
−
LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.
+
K731400 and psB1A2.
  
 
===References===
 
===References===
 +
(1) Chu L, Burgum A, Kolodrubetz D, and Holt SC. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun 1995 Nov; 63(11) 4448-55. <br>
 +
(2) Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, and Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol 2003 Aug; 62(2-3) 239-43. <br>
 +
(3) Wang CL, Maratukulam PD, Lum AM, Clark DS, and Keasling JD. Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 2000 Oct; 66(10) 4497-502. <br>

Latest revision as of 21:31, 26 October 2012

IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2460
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1559
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2490


Design Notes

This part was created by Gibson assembly starting from K731400. sfGFP was amplified by PCR from psB1A2. CysDes and sfGFP are separated by a short nucleotide linker that encodes for the amino acidic sequence GSGSGS.

Source

K731400 and psB1A2.

References

(1) Chu L, Burgum A, Kolodrubetz D, and Holt SC. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun 1995 Nov; 63(11) 4448-55.
(2) Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, and Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol 2003 Aug; 62(2-3) 239-43.
(3) Wang CL, Maratukulam PD, Lum AM, Clark DS, and Keasling JD. Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 2000 Oct; 66(10) 4497-502.