Difference between revisions of "Part:BBa K731400:Design"

(→‎Design Notes)
(→‎References)
 
(7 intermediate revisions by 2 users not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq promoter are in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
+
The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
  
−
===Source===
+
The nucleotide sequence of CysDes [https://parts.igem.org/Part:BBa_K731300 Part BBa_K731300] used to create this composite part has been codon optimised before Gene Synthesis by Genescript.
  
−
LacI+LacIq promoter comes from pET-21b plasmid.
+
===Source===
−
CysDes gene comes from the genome of Treponema denticola.
+
K731300 and K731600
  
 
===References===
 
===References===
 +
(1) Chu L, Burgum A, Kolodrubetz D, and Holt SC. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun 1995 Nov; 63(11) 4448-55. <br>
 +
(2) Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, and Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol 2003 Aug; 62(2-3) 239-43. <br>
 +
(3) Wang CL, Maratukulam PD, Lum AM, Clark DS, and Keasling JD. Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 2000 Oct; 66(10) 4497-502. <br>

Latest revision as of 21:30, 26 October 2012

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

The nucleotide sequence of CysDes Part BBa_K731300 used to create this composite part has been codon optimised before Gene Synthesis by Genescript.

Source

K731300 and K731600

References

(1) Chu L, Burgum A, Kolodrubetz D, and Holt SC. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun 1995 Nov; 63(11) 4448-55.
(2) Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, and Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol 2003 Aug; 62(2-3) 239-43.
(3) Wang CL, Maratukulam PD, Lum AM, Clark DS, and Keasling JD. Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 2000 Oct; 66(10) 4497-502.