Difference between revisions of "Part:BBa K731030:Design"
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===References=== | ===References=== | ||
| + | Jones-Mortimer, M.C.(1968). The control of sulphate reduction in Escherichia coli by O-acetyl-l-serine. Biochem. J. 106, 33. <br> | ||
| + | Kredich & Tomkins, 1966. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. Biochem. J. 241, 4955-4965. <br> | ||
| + | Denk, D., and A. Bock. 1987. L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. Gen. Microbiol. 133:515–525. <br> | ||
Latest revision as of 21:28, 26 October 2012
Inducible araC-pBAD promoter regulating M256I CysE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1909 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
K731201 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency.
Source
References
Jones-Mortimer, M.C.(1968). The control of sulphate reduction in Escherichia coli by O-acetyl-l-serine. Biochem. J. 106, 33.
Kredich & Tomkins, 1966. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. Biochem. J. 241, 4955-4965.
Denk, D., and A. Bock. 1987. L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. Gen. Microbiol. 133:515–525.