Difference between revisions of "Part:BBa K731500:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K731500 short</partinfo>
 
<partinfo>BBa_K731500 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part is composed of [https://parts.igem.org/Part:BBa_K731300 K731300] + a ''tac'' promoter followed by a
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''lac'' operator. ''lac'' operator sequence corresponds to plasmid pET21b one.
  
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''tac'' promoter and ''lac'' operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
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===Source===
 
===Source===
  
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pET21b
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[https://parts.igem.org/Part:BBa_K731300 BBa_K731300] part + P''tac'' & lacO from pET21b
  
 
===References===
 
===References===

Latest revision as of 10:30, 17 September 2012

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of K731300 + a tac promoter followed by a lac operator. lac operator sequence corresponds to plasmid pET21b one.

tac promoter and lac operator were amplified by PCR starting from a synthesized sequence composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.

Source

BBa_K731300 part + Ptac & lacO from pET21b

References