Difference between revisions of "Part:BBa K731300"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K731300 short</partinfo> | <partinfo>BBa_K731300 short</partinfo> | ||
− | This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG | + | This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG. It is a very useful device, it can be used as a ready to go cassette for cloning. It allows to control and regulate a gene of interest. |
+ | This part is an improvement of [https://parts.igem.org/Part:BBa_K314111 BBa_K314111]. | ||
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+ | This part has been characterised in part [https://parts.igem.org/Part:BBa_K731500 BBa_K731500] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 21:03, 26 September 2012
LacI + LacIq promoter (reverse)
This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG. It is a very useful device, it can be used as a ready to go cassette for cloning. It allows to control and regulate a gene of interest.
This part is an improvement of BBa_K314111.
This part has been characterised in part BBa_K731500 Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]