Difference between revisions of "Part:BBa K731020:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K731020 short</partinfo>
 
<partinfo>BBa_K731020 short</partinfo>
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===Design Notes===
 
===Design Notes===
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CysE was inserted after araC-pBAD in his vector, cutting the former with X&P and the latter with S&P.
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CysE WT ([https://parts.igem.org/Part:BBa_K731000 K731000]) was inserted after araC-pBAD ([https://parts.igem.org/Part:BBa_K731201 K731201]).
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===Source===
 
===Source===
  
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K731200, K731000
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[https://parts.igem.org/Part:BBa_K731201 K731201], [https://parts.igem.org/Part:BBa_K731000 K731000]
  
 
===References===
 
===References===

Latest revision as of 09:04, 23 August 2012

Inducible araC-pBAD promoter regulating WT CysE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

CysE WT (K731000) was inserted after araC-pBAD (K731201).

Source

K731201, K731000

References