Difference between revisions of "Part:BBa K731201:Design"

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===Design Notes===
 
===Design Notes===
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This part is similar to [http://https://parts.igem.org/Part:BBa_I0500 BBa_I0500], but it differs in several point mutations in the sequence.
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This is a mutated version of [https://parts.igem.org/Part:BBa_I0500 BBa_I0500]. The part was amplified directly from E. coli genome.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Lee et al., "Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the L-arabinose regulatory region of Escherichia coli", PNAS, Vol. 78, Num. 2, 752-756.

Latest revision as of 21:25, 26 October 2012

Arabinose inducible araC-pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This is a mutated version of BBa_I0500. The part was amplified directly from E. coli genome.

Source

E. coli genome

References

Lee et al., "Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the L-arabinose regulatory region of Escherichia coli", PNAS, Vol. 78, Num. 2, 752-756.