Difference between revisions of "Part:BBa K738000:Design"

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===Design Notes===
 
===Design Notes===
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The part is design with biobrick assmebly standard 10, which has standard prefix and suffix.
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Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation.
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===Safety Notes===
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The gene products of K738000 are RNAs and they will not be translated into proteins. As we know, RNA can be easily degraded, so our biobricks will not raise potentially safety problems.
  
 
===Source===
 
===Source===
  
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Dr. Camille J. Delebecque, the author of Organization of Intracellular Reactions with Rationally Designed RNA Assemblies, offer us D0 secquence in plsmid backbone pETDuet-BB.
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Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).
  
 
===References===
 
===References===
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Delebecque C. J., Lindner, A. B., Silver, P. A. & Aldaye, F. A. Organization of intracellular reactions with rationally designed RNA assemblies. Science http://dx.doi.org/10.1126/science.1206938 (2011).
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C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” ''Science'' 333, 470-474 (2011).

Latest revision as of 21:56, 26 September 2012

RNA Scaffold generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.

Safety Notes

The gene products of K738000 are RNAs and they will not be translated into proteins. As we know, RNA can be easily degraded, so our biobricks will not raise potentially safety problems.

Source

Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).

References

C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).