Difference between revisions of "Part:BBa K741006:Design"
MelodyChen (Talk | contribs) (→Design Notes) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K741006 short</partinfo> | <partinfo>BBa_K741006 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | We construct crogfp by overlap PCR. The primers we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 01:04, 3 October 2012
RBS-crogfp-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 81
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 875
Design Notes
We construct crogfp by overlap PCR. The primers we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.
Source
BBa_B0034 BBa_K741005 BBa_B0015