Difference between revisions of "Part:BBa K741002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We think it improved BBa_I13504(RBS-GFP-Terminator) in parts.igem. | |
| − | + | However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good. | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 19:20, 2 October 2012
plac-RBS-GFP-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
We think it improved BBa_I13504(RBS-GFP-Terminator) in parts.igem. However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good.
Source
BBa_R0010
BBa_I13504