Difference between revisions of "Part:BBa J100085:Experience"
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===Applications of BBa_J100085=== | ===Applications of BBa_J100085=== | ||
This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. | This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. | ||
| + | [[Image:CRISPR short.jpg|400px]] | ||
| + | *CRISPR and GFP should have no green fluorescence | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:06, 23 July 2012
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how you used this part and how it worked out.
Applications of BBa_J100085
This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should.
- CRISPR and GFP should have no green fluorescence
User Reviews
UNIQ81c28cc850a4ea46-partinfo-00000000-QINU UNIQ81c28cc850a4ea46-partinfo-00000001-QINU