Difference between revisions of "Part:BBa J100079"

 
 
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<partinfo>BBa_J100079 short</partinfo>
 
<partinfo>BBa_J100079 short</partinfo>
  
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This part combines Riboswitch D (Part J100065) and superfolder GFP optimized for E. coli (Part J100070).
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This part combines Riboswitch D ([https://parts.igem.org/Part:BBa_J100065 Part J100065]) and superfolder GFP optimized for E. coli ([https://parts.igem.org/Part:BBa_J100070 Part J100070]).  It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.
  
 
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Latest revision as of 14:17, 12 July 2012

Riboswitch and GFP

This part combines Riboswitch D (Part J100065) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172