Difference between revisions of "Part:BBa K917000:Experience"

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===Applications of BBa_K917000===
 
===Applications of BBa_K917000===
  
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Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.
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This part was used to create ars promoter plus cscA sucrose assimilation gene(BBa_K917001)and lac promoter plus sucrose hydrolase (cscA) (BBa_K917011). Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.
  
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[[Image:CONsample.jpg]] Control plate with no sugars
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<gallery widths=250px heights=250px style="border:none; text-align:center;background: white;">
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Image:CONsample.jpg|Control plate with no sugars
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[[Image:SUCsample.jpg]] Plate with sucrose as carbon source
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Image:SUCsample.jpg|Plate with sucrose as carbon source
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Image:GLUsample.jpg|Plate with glucose as carbon source
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[[Image:GLUsample.jpg]] Plate with glucose as carbon source
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</gallery>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:11, 1 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917000

This part was used to create ars promoter plus cscA sucrose assimilation gene(BBa_K917001)and lac promoter plus sucrose hydrolase (cscA) (BBa_K917011). Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.

  • Control plate with no sugars

  • Plate with sucrose as carbon source

  • Plate with glucose as carbon source

User Reviews

UNIQ6eccde48fadc43b8-partinfo-00000001-QINU UNIQ6eccde48fadc43b8-partinfo-00000002-QINU