Difference between revisions of "Part:BBa J100065:Design"

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===Design Notes===
 
===Design Notes===
−
None
+
Designed to permit translation in the presence of theophylline.
  
 
===Source===
 
===Source===
  
−
http://www.ncbi.nlm.nih.gov/pubmed?term=Synthetic%20Riboswitches%20That%20Induce%20Gene%20Expression%20in%20Diverse%20Bacterial%20Species
+
Synthetic part taken from reference below.
  
 
===References===
 
===References===
 +
 +
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed

Latest revision as of 13:52, 12 June 2012

Synthetic Riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal NotI site found at 7
    Illegal NotI site found at 201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 187


Design Notes

Designed to permit translation in the presence of theophylline.

Source

Synthetic part taken from reference below.

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed