Difference between revisions of "Part:BBa K896999:Design"

 
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<partinfo>BBa_K896999 short</partinfo>
 
<partinfo>BBa_K896999 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Many design considerations were taken into account when creating this sequence
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Primers used in the PCR:
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X174_E_FP: gcttctag atggtacgctggactttgt (TM:55C)
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X174_E_RP: gct ctgcagcggccgctactagta tcactccttccgcacg (TM:55C)
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Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.
  
 
===Source===
 
===Source===
  
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From where
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PCR'd from &Phi;X174 wildtype phage.
  
 
===References===
 
===References===
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[Zheng09] Zheng ''et al'' (2009) Purification and Functional Characterization of &Phi;X174 Lysis Protein E, Biochemistry, 48 (22), pp 4999–5006

Latest revision as of 04:20, 5 April 2012

ΦX174; E (lysis) gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used in the PCR: 

X174_E_FP: gcttctag atggtacgctggactttgt (TM:55C)
X174_E_RP: gct ctgcagcggccgctactagta tcactccttccgcacg (TM:55C)

Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.

Source

PCR'd from ΦX174 wildtype phage.

References

[Zheng09] Zheng et al (2009) Purification and Functional Characterization of ΦX174 Lysis Protein E, Biochemistry, 48 (22), pp 4999–5006