Difference between revisions of "Part:BBa J176121"
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* Must be co-transfected with a plasmid expressing Flp recombinase | * Must be co-transfected with a plasmid expressing Flp recombinase | ||
* Host cells must carry a FRT target site | * Host cells must carry a FRT target site | ||
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Latest revision as of 23:01, 19 December 2011
V0200
FRT-marked mammalian transfection vector (from Invitrogen pcDNA5 FRT/TO); no promoter; amp, hygro
Usage and Biology
- Used to create a stable insert into a mammalian chromosome
- Must be co-transfected with a plasmid expressing Flp recombinase
- Host cells must carry a FRT target site
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1238
Illegal XbaI site found at 970
Illegal SpeI site found at 329
Illegal PstI site found at 1328 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1238
Illegal SpeI site found at 329
Illegal PstI site found at 1328
Illegal NotI site found at 309 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1238
Illegal BglII site found at 47 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1238
Illegal XbaI site found at 970
Illegal SpeI site found at 329
Illegal PstI site found at 1328 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1238
Illegal XbaI site found at 970
Illegal SpeI site found at 329
Illegal PstI site found at 1328
Illegal NgoMIV site found at 831
Illegal NgoMIV site found at 2093
Illegal AgeI site found at 396 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 378
Illegal BsaI.rc site found at 3621
Illegal SapI site found at 2538