Difference between revisions of "Part:BBa J176008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | * Include within the ORF of a protein that you need | + | * Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor) |
| − | * | + | * Fluorophore-NLS fusions are handy for making glowing nuclei |
| + | * Very effective in cultured human cells | ||
| − | PKKKRKV motif from the | + | PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. In our hands, the localization appears to be very efficient in U2OS and HEK293 cells (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges. |
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Latest revision as of 17:44, 9 October 2012
NLS
SV40 nuclear localization sequence
Usage and Biology
- Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor)
- Fluorophore-NLS fusions are handy for making glowing nuclei
- Very effective in cultured human cells
PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. In our hands, the localization appears to be very efficient in U2OS and HEK293 cells (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]