Difference between revisions of "Part:BBa J176136"
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Latest revision as of 04:03, 11 December 2011
HPK-RFP-miRts BCL2
Red fluorescent protein designed to be repressed by microRNAs (miR's) from luciferase (artificial) and miR-34 (natural)
Alias: DF4
Usage and Biology
Double mCherry red fluorescent protein that enters the nucleus (via the NLS tag) and binds to Gal4 DNA sequences (via Gal4DB). It is designed to be repressed by either miR-34 (via miRts BCL2) or miR-luc (via miRts luc).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3106
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 764
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 683