Difference between revisions of "Part:BBa M36304"

 
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<partinfo>BBa_M36304 short</partinfo>
 
<partinfo>BBa_M36304 short</partinfo>
  
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The part is (enter text here).
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Constitutive promoter bounded by four FseI sites. The expected operational result is that the promoter will be spliced out of a plasmid when FseI is present.
  
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===Usage and Biology===
 
===Usage and Biology===
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Place this part next to a gene of interest so that the gene is constitutively expressed until the restriction endonuclease FseI is present. Effectively, this part will work to knock out a gene.
  
 
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Latest revision as of 07:22, 10 December 2011

Constitutive promoter with FseI restriction sites

Constitutive promoter bounded by four FseI sites. The expected operational result is that the promoter will be spliced out of a plasmid when FseI is present.

Usage and Biology

Place this part next to a gene of interest so that the gene is constitutively expressed until the restriction endonuclease FseI is present. Effectively, this part will work to knock out a gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 93
    Illegal NgoMIV site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]