Difference between revisions of "Part:BBa M36301:Design"

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===Design Notes===
 
===Design Notes===
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This site was designed to guard against DNA methylation.
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The spacers surrounding the restriction sites were designed to guard against DNA methylation.
  
 
===Source===
 
===Source===
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N/A
 
  
 
===References===
 
===References===
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FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence.
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    Nelson, J.M., Miceli, S.M., Lechevalier, M.P. and Robers, R.J. 1990. FseI, a new type II restriction
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    endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res., 18, 2061-2064.

Latest revision as of 07:26, 10 December 2011

Dual FseI restriction sites bounded by spacers


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacers surrounding the restriction sites were designed to guard against DNA methylation.

Source

References

FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence. 

    Nelson, J.M., Miceli, S.M., Lechevalier, M.P. and Robers, R.J. 1990. FseI, a new type II restriction 
    endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res., 18, 2061-2064.