Difference between revisions of "Part:BBa M36282"

Latest revision as of 23:34, 8 December 2011

5' Bicistronic UTR, includes ATG start codon

This part is a high expressing 5' UTR based on bicistronic junction architecture. The first RBS is strong and drives expression of short leader cistron. The second RBS is internal to the first cistron and controls translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_M36282 short</partinfo>
-This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG.
+This part is a high expressing 5' UTR based on bicistronic junction architecture. The first RBS is strong and drives expression of short leader cistron. The second RBS is internal to the first cistron and controls translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
 <!-- Add more about the biology of this part here