Difference between revisions of "Part:BBa J176013:Design"

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===Design Notes===
 
===Design Notes===
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TBA
 
−
 
  
 +
No modifications. Exactly the same as Beerli et al.'s (1998) good old VP64.
  
 
===Source===
 
===Source===
  
−
TBA
+
I first started using VP64 when I was a postdoc in Pam Silver's lab at Harvard Medical School (2008 - 2011). VP64 was a part of the lab collection.
  
 
===References===
 
===References===
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'''(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.'''<br>
 
'''(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.'''<br>
−
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors<br>
+
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML, amino acids 437-447; activity was compared to activation domains from other transcription factors<br>
 +
 
 +
'''(1998) Beerli RR, Segal DJ, Dreier B, Barbas CF 3rd. Toward controlling gene expression at will: Specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14628-33.'''<br>
 +
Note: Earliest report of the construction and use of the VP16 fusion "VP64" ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) which is four copies of VP16 with glycine-serine linkers.<br>
 +
 
  
−
'''(2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.'''<br>
+
Since 1998, VP64 had been used again by the authors (Beerli and colleagues) in several publications, and gained popularity as it was used by many other groups to make custom synthetic transcription factors.
−
Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in 2005
+

Latest revision as of 02:44, 22 November 2011

VP64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No modifications. Exactly the same as Beerli et al.'s (1998) good old VP64.

Source

I first started using VP64 when I was a postdoc in Pam Silver's lab at Harvard Medical School (2008 - 2011). VP64 was a part of the lab collection.

References

In order of date published:

(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML, amino acids 437-447; activity was compared to activation domains from other transcription factors

(1998) Beerli RR, Segal DJ, Dreier B, Barbas CF 3rd. Toward controlling gene expression at will: Specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14628-33.
Note: Earliest report of the construction and use of the VP16 fusion "VP64" ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) which is four copies of VP16 with glycine-serine linkers.


Since 1998, VP64 had been used again by the authors (Beerli and colleagues) in several publications, and gained popularity as it was used by many other groups to make custom synthetic transcription factors.