Difference between revisions of "Part:BBa K511819"

Latest revision as of 16:18, 10 May 2013

Inducible Yellow Fluorescent Protein Generator (UAS-Gal4-Citrine) MammoBlock Device

This MammoBlock composite device produces the yellow/green fluorescent protein Citrine when induced with Gal4 transactivator variants and otherwise produces Citrine at a low, basal (OFF) level.

Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels.

As can be clearly seen, Hef1a:GV16 results in significant activation of the UAS promoter. This results in the observed increases in mean fluoresence compared to control populations. With the exception of UAS:eBFP2, we see more than 20-fold increase in mean fluorescence.

Sequence and Features