Difference between revisions of "Part:BBa K553008:Experience"
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bacteria Intra-strain communication: | bacteria Intra-strain communication: | ||
| − | An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule. The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria. | + | An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (Strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B). |
The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8. | The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8. | ||
OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM | OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM | ||
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[[Image:Coculture_assay.jpg]] | [[Image:Coculture_assay.jpg]] | ||
The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes. | The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes. | ||
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| + | Here the HPLC-MS/MS analysis of the co-culture of the strain A and B. | ||
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| + | Even a very linle production of OC12 is able to induce a good production of OC8. | ||
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| + | [[Image:Ms_co-culture_.png]] | ||
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| + | OC8 signal @ 10 min; OC12 signial @ 12 min | ||
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| + | blue: standards: 1uM 5uL loaded | ||
| + | black: medium background, 40x concentrated organic extract 12,5 uL loaded | ||
| + | red: co-culture sample, 40x concentrated organic extract 12,5 uL loaded | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:35, 1 November 2011
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Applications of BBa_K553008
UNITS_Trieste Synbiome project
bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (Strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B).
The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.
OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM
The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.
Here the HPLC-MS/MS analysis of the co-culture of the strain A and B.
Even a very linle production of OC12 is able to induce a good production of OC8.
OC8 signal @ 10 min; OC12 signial @ 12 min
blue: standards: 1uM 5uL loaded black: medium background, 40x concentrated organic extract 12,5 uL loaded red: co-culture sample, 40x concentrated organic extract 12,5 uL loaded
User Reviews
UNIQe42de8e869514baa-partinfo-00000002-QINU UNIQe42de8e869514baa-partinfo-00000003-QINU